闵友江，程立红，姚海华，杨柳，闵志云，裴　佳

（江西中医药大学附属医院，南昌，江西330006）

摘要：目的：探讨“三通针法”治疗对大鼠脊髓损伤后p75 神经营养素受体(p75NTR) mRNA及蛋白表达的影响。方法：72只Sprague-Dawley 雌性大鼠随机分成假手术组(A，n=8)和造模组(n=64)。造模组大鼠采用改良Allen 重物打击法制作脊髓损伤大鼠模型，造模成功后存活48 只大鼠，随机分为模型组(B，n=12)、电针组(C，n=12)、阻断剂NEP1-40 组(D，n=12)、电针+阻断剂NEP1-40 组(E，n=12)。电针治疗取大椎、腰阳关及双侧次髎、足三里，选择疏密波，持续时间20 min，每天1 次。分别于治疗后7 d、14 d 提取损伤处脊髓组织，采用实时荧光定量PCR、原位杂交、Western blotting 方法分别检测p75NTR mRNA及蛋白的表达，采用BBB评分评估大鼠后肢活动功能。结果：BBB评分显示，治疗后各组评分均较B组提高，且E组BBB评分分别高于C组和D组(P<0.05)，各组14 d 评分均高于7 d (t>2.623, P<0.05)。脊髓损伤后，各治疗组与B组比较，脊髓组织中p75NTR mRNA及蛋白的表达均降低(P<0.05)；C组、D组和E组之间无显著性差异(P>0.05)。结论：“三通针法”电针能改善脊髓损伤大鼠后肢运动功能，抑制脊髓损伤后p75NTR的表达活动，这可能是电针治疗脊髓损伤的机制之一。

关键词： 脊髓损伤；电针；神经再生；p75 神经营养素受体；大鼠

Effect of Santong Electroacupuncture on Expression of p75 Neurotrophin Receptor in Rats with Spinal Cord Injury

MIN Youjiang, CHENG Lihong, YAO Haihua, YANG Liu,

 MIN Zhiyun, PEI Jia

(Affiliated Hospital of Jiangxi University of Traditional Chinese Medicine,

 Nanchang, Jiangxi 330006, China)

Abstract: Objective: To investigate the effect of Santong electroacupuncture (EA) on mRNA and protein expression of p75 neurotrophinreceptor (p75NTR) in rats with spinal cord injury (SCI).  Methods: A total of 72 female Sprague-Dawley rats were randomly assigned to sham operation group (group A, n=8) and model group (n=64). In the model group, Allen's method was used to make SCI rats model, in which 48 survived model rats were further subdivided into model control group (group B, n=12), EA group (group C, n=12), inhibitor Nogo extra cellular peptide residues 1-40 (NEP1-40) group (group D, n=12) and EA+inhibitor NEP1-40 group (group E, n=12) according to design proposal. The treatment groups were electroacupunctured on Dazhui (GV14) and Yaoyangguan (GV3), bilateral Ciliao (BL32) and Zusanli(ST36) with loose-tight wave, for 20 minutes every day. After 7 and 14 days of treatment, injured spinal cord tissue was extracted for detecting. The mRNA and protein expression of p75NTR was detected by real-time fluorescent quantitative PCR, in situ hybridization and Western blotting respectively. The hind limb motor function was assessed with Basso-Beattie-Bresnahan (BBB) score. Results:The BBB score increased in the treatment groups compared with group B, and was higher in group E than in groups C and D (P<0.05), as well as on the 14th day than on the 7th day in all the treatment groups (t>2.623, P<0.05). The mRNA and protein expression of p75NTR in spinal cord tissues decreased in the treatment groups compared with group B (P<0.05), and no significant difference was found among the treatment groups (P>0.05). Conclusion:Santong elerctroacupuncture treatment could improve the hind limb motor function, which may associate with inhibition of the mRNA and protein expression of p75NTR in rats after SCI.

Key words:spinal cord injury, electroacupuncture,neural regeneration, p75 neurotrophin receptor, rats