<p style="text-align: center;">闵友江，程立红，姚海华，杨柳，闵志云，裴　佳</p><p style="text-align: center;">（江西中医药大学附属医院，南昌，江西330006）</p><p style="text-align: justify;">摘要：目的：探讨“三通针法”治疗对大鼠脊髓损伤后p75 神经营养素受体(p75NTR) mRNA及蛋白表达的影响。方法：72只Sprague-Dawley 雌性大鼠随机分成假手术组(A，n=8)和造模组(n=64)。造模组大鼠采用改良Allen 重物打击法制作脊髓损伤大鼠模型，造模成功后存活48 只大鼠，随机分为模型组(B，n=12)、电针组(C，n=12)、阻断剂NEP1-40 组(D，n=12)、电针+阻断剂NEP1-40 组(E，n=12)。电针治疗取大椎、腰阳关及双侧次髎、足三里，选择疏密波，持续时间20 min，每天1 次。分别于治疗后7 d、14 d 提取损伤处脊髓组织，采用实时荧光定量PCR、原位杂交、Western blotting 方法分别检测p75NTR mRNA及蛋白的表达，采用BBB评分评估大鼠后肢活动功能。结果：BBB评分显示，治疗后各组评分均较B组提高，且E组BBB评分分别高于C组和D组(P&lt;0.05)，各组14 d 评分均高于7 d (t&gt;2.623, P&lt;0.05)。脊髓损伤后，各治疗组与B组比较，脊髓组织中p75NTR mRNA及蛋白的表达均降低(P&lt;0.05)；C组、D组和E组之间无显著性差异(P&gt;0.05)。结论：“三通针法”电针能改善脊髓损伤大鼠后肢运动功能，抑制脊髓损伤后p75NTR的表达活动，这可能是电针治疗脊髓损伤的机制之一。</p><p style="text-align: justify;">关键词： 脊髓损伤；电针；神经再生；p75 神经营养素受体；大鼠</p><p style="text-align: center;"><strong>Effect of Santong Electroacupuncture on Expression of p75 Neurotrophin Receptor in Rats with Spinal Cord Injury</strong></p><p style="text-align: center;">MIN Youjiang, CHENG Lihong, YAO Haihua, YANG Liu,</p><p style="text-align: center;">&nbsp;MIN Zhiyun, PEI Jia</p><p style="text-align: center;">(Affiliated Hospital of Jiangxi University of Traditional Chinese Medicine,</p><p style="text-align: center;">&nbsp;Nanchang, Jiangxi 330006, China)</p><p style="text-align: justify;">Abstract: Objective: To investigate the effect of Santong electroacupuncture (EA) on mRNA and protein expression of p75 neurotrophinreceptor (p75NTR) in rats with spinal cord injury (SCI).&nbsp; Methods: A total of 72 female Sprague-Dawley rats were randomly assigned to sham operation group (group A, n=8) and model group (n=64). In the model group, Allen&#39;s method was used to make SCI rats model, in which 48 survived model rats were further subdivided into model control group (group B, n=12), EA group (group C, n=12), inhibitor Nogo extra cellular peptide residues 1-40 (NEP1-40) group (group D, n=12) and EA+inhibitor NEP1-40 group (group E, n=12) according to design proposal. The treatment groups were electroacupunctured on Dazhui (GV14) and Yaoyangguan (GV3), bilateral Ciliao (BL32) and Zusanli(ST36) with loose-tight wave, for 20 minutes every day. After 7 and 14 days of treatment, injured spinal cord tissue was extracted for detecting. The mRNA and protein expression of p75NTR was detected by real-time fluorescent quantitative PCR, in situ hybridization and Western blotting respectively. The hind limb motor function was assessed with Basso-Beattie-Bresnahan (BBB) score. Results:The BBB score increased in the treatment groups compared with group B, and was higher in group E than in groups C and D (P&lt;0.05), as well as on the 14th day than on the 7th day in all the treatment groups (t&gt;2.623, P&lt;0.05). The mRNA and protein expression of p75NTR in spinal cord tissues decreased in the treatment groups compared with group B (P&lt;0.05), and no significant difference was found among the treatment groups (P&gt;0.05). Conclusion:Santong elerctroacupuncture treatment could improve the hind limb motor function, which may associate with inhibition of the mRNA and protein expression of p75NTR in rats after SCI.</p><p>Key words:spinal cord injury, electroacupuncture,neural regeneration, p75 neurotrophin receptor, rats</p>